Opposition Leader Matthew Guy moved the motion over the misuse of $388,000 of taxpayer money to partially fund Labor campaign staff during the 2014 election.

But the motion was lost in a vote of 49 to 33 on Tuesday night.

"This government is corrupt, they rorted taxpayers money, they tried to cover it up," Mr Guy told the Legislative Assembly during Tuesday's lengthy debate.

He cited a series of rorts Labor MPs had been caught out on since coming to power - the misuse of second residence allowances, chauffeuring of dogs in ministerial cars and suspected branch stacking through printing invoices.

Deputy Premier James Merlino labelled the motion as "desperate" and suggested Victorian Liberals could face a similar leadership spill to their federal colleagues.

Labor is under police investigation over its rorts-for-votes scandal.

The probe was sparked after Ombudsman Deborah Glass' March report into the scandal, which described the scheme as an "artifice", but also concluded MPs involved did not deliberately deceive.

The government has referred the Liberal-Nationals to Ms Glass for investigation over alleged potential knowledge or inappropriate involvement with embezzler and former Liberal director Damien Mantach.

Labor has also referred 18 current and former Liberal and National MPs to police, alleging they also misused electorate staff at the last election.

Australian Associated Press